As a kind of bionic robot fish,bionic killer whale played an important role in ocean development and scientific investigation.Based on the theory of flapping wing movement,the biomimetic killer whale was designed in the way of fish propulsion,the load was nonlinear,time-varying and strongly coupled.The bionic killer whale‘s force model was established through the analysis of the bionic killer whale’s straight line and turning movement.The instantaneous load’s text file of bionic killer whale was generated by MATLAB,the instantaneous load was successfully introduced into the rigid-flexible coupling model by ADAMS spline function,which provided a method and condition for qualitative and quantitative analysis of bionic killer whale’rigid-flexible coupling reliability analysis.The results show that MATLAB can accurately simulate the periodic change of the bionic killer whale’s instantaneous load,providing a clear quantitative data for the bionic killer whale’s reliability research.